Christoph Zimmermann has been ruled out for four to six weeks following a hamstring injury picked up in training, Daniel Farke has confirmed.
Lukas Rupp and Adam Idah are also not available for Friday's trip to Preston North End, the latter continuing to recover from his hernia surgery.
A number of City players featured for their countries on Wednesday night, and Farke explained the complications surrounding those players in his pre-match press conference.
"All of the lads who played yesterday, I can't confirm if they're ready to go," he said. "They have to arrive here in time and return negative tests."
The City head coach also confirmed that those who had finished their international duties before Wednesday had travelled back to Norwich and returned negative tests, so are available for Preston.